Anticancer effect of ginger extract against pancreatic cancer. Plants that are used as traditional medicine represent a relevant pool for selecting plant candidates that may have anticancer properties. Anticancer activity the seed and root extracts of h. Moreover, it showed high anticancer activity by growth inhibition in the. Antioxidant, anticancer activity and phytochemical. The extract of ginger zingiber officinale roscoe and its major pungent components, 6shogaol and 6gingerol, have been shown to have an antiproliferative effect on several tumor cell lines. Evaluation of antimitotic activity of solanum torvum using. Chromatograms of sfe extracts of silver birch upper and downy birch. Many plantderived products have been reported to exhibit potent antitumor activity against several rodent and human cancer cell lines 3. The evaluation of the anticancer activity of plant extracts is essential for safe treatment. The antioxidant activity of spirulina water extracts was measured based on the scavenging activity of the stable 1, 1diphenyl 2picrylhyorazyl dpph free radical according to the method described by choi et al. The ec50 of methanolic seed extract mse and root extract table 1.
Forty eight extracts from 38 medicinal plants distributed into 19 different plant families were evaluated for cytotoxic activity on mammalian cells llcmk2 cells as presented in table 1. The most active extracts were further screened on mcf7. Gaidhani et alevaluation of plant extracts for standardization and anticancer activity 683 activity against cells derived from human carcinoma of nasopharynx kb4 and also acts as a potent antiproliferative activity against pancreatic cancer. Hence, these meristamatic cells can be used for preliminary screening of drugs with anticancer activity. We tested a variety of cancer cell lines originating from various human tumors including hematopoietic tumors such as t cell lymphoma and leukemia, as well as solid tumors such as colon, renal, breast, bladder, endometrial, and others table 1. Anticancer activity of ruellia britoniana flower on. Anticancer activity of essential oils and other extracts. Evaluation of traditionally used medicinal plants for anticancer. Evaluation of antiros and anticancer properties of. Plant extract also prolong the life of mice bearing a transplantable lymphocytic leukemia. Research article evaluating medicinal plants for anticancer.
The aim of this study was to assess the efficacies of three plant extracts for their potential antioxidant and antiinflammatory activity in primary human skin fibroblasts. However, current research about the mechanisms of the anticancer potential of va. The dpph radical scavenging assay exhibited higher antioxidant activity ic50 9. Plant extracts 5, 10, and 11 were tested also on primary lymphocytes from healthy control donors and showed no killing activity on these cells figure 2c. It is an eye opening for us to study on anticancer activity of methanol extract of e. Some extracts also appears to be good sources of potential antioxidant and anticancer agents. Flavonoidrich extracts from the mature roots of scutellaria baicalensis have been shown to exhibit antiproliferative effects on various cancer cell lines. The aim of this study was to determine the invitro antiros and invivo anticancer activities of tabebuia pallida leaves abbreviated as tpl against ehrlichs ascite carcinoma eac cells. In vitro anti cancer activity of mangrove plant extracts at various concentrations 10 to 200ugml was studied against the chosen cell line using mtt assay 34, 5dimethylthiazol2yl2, 5diphenyltetrazolium bromide. Here, we demonstrate that the ethanolextracted materials of ginger suppressed cell cycle progression. In vitro anti cancer activity of mangrove plant extracts at various. The collected plants were then evaluated for cytoxicity. Breast cancer is currently the leading cause of cancerrelated deaths among women globally.
Vernonia amygdalina va is a woody shrub reported to have not only diverse therapeutic effects but also anticancer properties. In this study, the ethnomedicinal approach was used to select several medicinal plants native to nigeria, on the basis of their local or traditional uses. The wst1 assay was used to test the extracts on the proliferation of the prostate cancer cell line, pc3. Evaluation of venezuelan medicinal plant extracts for antitumor and antiprotease activities peter g. The aim of this study was to evaluate the anticancer activity of morus nigra leaves against human cervical cancer cell line hela. Vinca r osea alkaloids, vinblastine and vincristine, are one of the most potent anticancer drugs known. Plant extract 5 urtica membranacea showed particularly strong anticancer capabilities since it. During the present investigation, crude methanol extracts of selected mangrove plants were prepared using soxhlet apparatus.
The anticancer activity of the different extracts of fruits of piper longum on human lung epithelial adenocarcinoma cell line hcc827 has been assessed in vitro using 34,5dimethylthiazol2yl2,5diphenyl tetrazolium bromide mtt assay. As previously mentioned polyphenols can inhibit or alter the regulation of proteins and other agents which may be contributing to the survival of cancer cells. This effect differs according to concentrations used. It could also provide an indication of possible cytotoxic properties of the tested plant extracts. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. The results revealed that the polarity of the extraction solvent affects the tpc, tfc, and aa. The inhibitory activity of plant extracts on mmp9 bacterial. Anticancer activity is the effect of natural and synthetic or biological and chemical agents to reverse, suppress or prevent carcinogenic progression. Anticancer activities of the extracts were determined by the mtt colorimetric assay in the prostate cancer cell line pc3. Antifungal activity of different extracts of lantana camara stem against a. Aromatic plants have a long and significant history in the traditional medicine of many countries.
Evaluation of some plant extracts for standardization and anticancer activity article pdf available in indian journal of traditional knowledge 124october 20. Evaluation of the anticancer potential of six herbs. The anticancer activity showed leaf hexane extract ic50 values 8. It enables identification of the intrinsic toxicity of the plant and the effects of acute overdose. Our results suggest that whole plant extracts are promising anticancer reagents. The anticancer activities of vernonia amygdalina extract.
Oct, 2011 numerous reports have identified therapeutic roles for plants and their extracts and constituents. Antioxidants, streptomyces pds1, saccharomyces cerevisiae, mtt assay, anticancerous activity. Evaluation of antiros and anticancer properties of tabebuia. Anticancer activity of plant extracts containing flavonoids fraction. The cervical hela and liver cancer hepg2 cell lines were used to screen the in vitro anticancer challenging with the b. However, the anticancer activity of the ginger extract in pancreatic cancer is poorly understood. Results effect of the plant extracts on cytotoxicity in different cancer cell lines the 40 extracts from different parts of the 17. The data suggest that tc methanolic extract exhibited potent cytotoxic activity with an ic50 value less than 100. Six herbs in the plant genetics conservation project that have been used as complementary medicines were chosen on the basis of their medicinal value, namely terminalia mucronata, diospyros winitii, bridelia insulana, artabotrys harmandii, terminallia triptera, and croton oblongifolius.
Investigations on three different cancer cell lines c2cl2, 3t3l1, hela revealed direct inhibitory effect of the extracts. Antiproliferative activity of some medicinal plants on human breast. Research article evaluating medicinal plants for anticancer activity elishasolowey, 1 michallichtenstein, 1 sarahsallon, 2 helenapaavilainen, 2 elainesolowey, 3 andhayalorberboumgalski 1 department of biochemistry and molecular biology, institute for medical research israelcanada imric. In the current study, we investigated the presence of bioactive compounds from each part of e. Apr 15, 2019 the aim of this study was to determine the invitro antiros and invivo anticancer activities of tabebuia pallida leaves abbreviated as tpl against ehrlichs ascite carcinoma eac cells. Pdf evaluation of some plant extracts for standardization. Cell death caused by the whole plant extracts is via apoptosis. They can be readily administered orally as part of patients dietary intake 2930. The low toxicity of water extracts of the three plants and the antimicrobial activity recorded validates the use of these extracts in traditional medicine.
The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. Anticancer activity of the plant extracts were also tested on hos and khos osteosarcoma cell lines. In vitro antioxidant and anticancer activity of leea. Thus, plant extracts 5, 10, and 11 exhibited time and concentrationdependent anticancer activity while having no effect on primary cell cultures from healthy humans. Aug 16, 2006 flavonoidrich extracts from the mature roots of scutellaria baicalensis have been shown to exhibit antiproliferative effects on various cancer cell lines. Aqueous extracts and formulations of white tea, witch hazel and rose were subjected to assays to measure anticollagenase.
Whole plant extracts ethanol extracted were prepared, coded as plant extract numbers 117, and tested for their potential as anticancer agents. Anticancer activity of extracts derived from the mature. Medicinal plants and plant extracts scanned pdf, 2 mb. Nov, 2014 plant extracts 5, 10, and 11 were tested also on primary lymphocytes from healthy control donors and showed no killing activity on these cells figure 2c. It was found that both tpc and aa are highest for plant extracted with 80% ethanol, followed by. Camptothecin was introduced to clinical trials by the national cancer institute in the 1970s, but withdrawn soon because of reports of severe bladder toxicity. Anticancer activity, antioxidant activity, and phenolic.
Secondary metabolites coming from naturally occurring material are useful source of bioactive molecule. Research article evaluating medicinal plants for anticancer activity elishasolowey, 1 michallichtenstein, 1 sarahsallon, 2 helenapaavilainen, 2 elainesolowey, 3 andhayalorberboumgalski 1 department of biochemistry and molecular biology, institute for medical research israelcanada imric, hebrew university hadassah medical school, jerusalem. In vitro anticancer screening of 24 locally used nigerian. Anticancer, antibacterial, and antifungal activities of arum. The effects of plant leaves extract on cancer cells was studied. Anticancer activity of essential oils and other extracts from.
Pdf anticancer activity of morus nigra leaves extract. In the present study, the various extracts viz aqueous, ethanol, chloroform and petroleum ether of biophytum sensitivum whole plant were evaluated for the anticancer activity against cancer cell lines. Pdf anticancer activity of morus nigra leaves extract researchgate. However, as described in sections 3 to 5, a number of agents are in preclinical development. Anticancer, antioxidant activities and gcms analysis of. This is the reason why the study of plant extracts as therapeutic agents is becoming more and more current nowadays. Anticancer activity using mcf7 human mammary gland breast adenocarcinoma cell lines december, 2011 drug invention today, 2011, 312, 290296 291 this division is similar to the above mentioned cancer division in humans. Original article antioxidant, antimicrobial and anticancer. Evaluation of the cytotoxic activity of extracts from. This study aims to evaluate the potential anticancer activity of 50% ethanolwater. Selected plants have been explored for biological activity and further investigations into anticancer activity of the plants showing promising activity, must be undertaken. Determination of dna protecting activity the hydroxyl radical scavenging activities of the extracts were estimated using plasmid nicking assay lee, et al. The anticancer activities of vernonia amygdalina extract in. Issn 23203862 evaluation of anticancer activity of stem.
Petroleum ether 4060 c, chloroform, ethyl acetate and methanol 80% extracts of c. Antioxidant, anticancer and antimicrobial activities of. The cytotoxic activity of the microalgae extracts is shown in table 3. Medicinal plants and plant extracts scanned pdf, 2 mb author. To evaluate the cytotoxic properties of granulocystopsis sp.
Newman encyclopedia of life support systems eolss reached the stage of general use. In vitro anticancer activity of microbial isolates from. In vitro anticancer activity of methanolic extract of. One of the best approaches in the search of anticancer agents from plant sources is the. Notably, medicinal plant extracts may be a potential source for treatments of breast cancer. In vitro anticancer activity studies the extracellular and intracellular extracts prepared were subjected to in vitro cytotoxicity screening on hela cells by the mtt 34, 5dimethylthiazol2yl2, 5diphenyltetrazolium bromide method. Recent discoveries of anticancer flavonoids sciencedirect. Anticancer activity of extracts derived from the mature roots. Evaluation of the anticancer potential of six herbs against a.
Anticancer activity of carica papaya extracts in and. The crude ethanolic extract cee 70% vv was fractionated successively with different solvents to get petroleum ether pef, chloroform chf, ethyl acetate eaf and aqueous fraction aqf. Matrix metalloproteinase 9 activity assay was performed in 50mm tris hcl buffer, ph 7. We assessed the ability of an ethanolic extract of s. In recent years owing to the fear of side effects people prefer to use natural plant products for cancer treatment. Antioxidant and anticancer activity of spirulina platensis. Evaluating medicinal plants for anticancer activity. Plantderived anticancer agents in clinical use figure 1. Plant extract 5 urtica membranacea showed particularly strong anticancer capabilities since it inhibited actual tumor progression in a breast adenocarcinoma mouse model. The killing activity was specific toward tumor cells, as the plant extracts had no effect on primary cultures of healthy human cells. Study of antioxidant and anticancer activity of natural. Plants are screened for treatment of many ailments including cancer because they possess certain potential constituents which are effective for treatment.
Nowadays, there is an increasing interest in investigating the biological properties of aromatic plant extracts mainly due to their diversity, high availability, and low toxicity. The mtt assay results were used to evaluate the anticancerous activity of the extract. Antioxidant and potential antiinflammatory activity of. Oct 01, 2015 other studies have looked at flavonoid extracts from fern species and found that even in low concentrations they still demonstrate high percentage of anticancer activity 23.
Plant extract 5 urtica membranacea showed particularly strong anticancer capabilities since it inhibited actual tumor progression in a. The nature of cytotoxic activity is found to be apoptotic rather than necrosis by hoechst test. The dpph radical scavenging activity is one of the most widely used methods for screening the antioxidant activity of plant extracts. The anticancer properties of phytochemical extracts from salvia plants yuanyuan jiang,1,2 li zhang,2 hp vasantha rupasinghe1,3 1department of environmental sciences, faculty of agriculture, dalhousie university, truro, ns, canada. Anticancer effect of ginger extract against pancreatic. Analysis of antioxidant and anticancer potentials of. Cell lines derived from primary and recurrent brain tumors from the same patient and cells selected for. Thus, we have made an attempt to use this herbal plant to check the efficacy against prostate cancer cell lines. Antioxidant and in vitro cytotoxic activity of extracts of. In vitro antioxidant and anticancer activity of leea indica leaf extracts. Plant extracts screened for their antiproliferative activity by mtt assay as can be seen in table 1 and 2, the ethanol extract of tubers of decalebis arayalpatra showed potential antiproliferative and cytotoxicity against all the screened cell. The compound 1 is being reported for the first time from this plant. Anti cancer activity of four selected mangrove plants bruguiera gymnorrhiza, aegiceras corniculatum, aegialitis rotundifolia and lumnitzera racemosa against hepg2 cell line was studied. The anticancer activity of four medicinally used plants against cervical and epidermoid cancer.
Plantderived anticancer agents in clinical use figure 1 3. Anticancer, antioxidant and antimicrobial sreening of. Pdf in vitro and in vivo methods for anticancer activity. The anticancer properties of phytochemical extracts from.
Anticancer, antibacterial, and antifungal activities of. In vitro antioxidant and anticancer activity of leea indica. Evaluation of some plant extracts for standardization and. The most active extracts were further screened on mcf7 cells by mtt assay. The ethanolic extracts exhibits hepg2 anticancer activity at minimum concentration of 30. The potency of plant extracts to inhibit the cancerous growth was recordedin terms of decrease in. Also, being naturally derived compounds from plants they are generally more tolerated and nontoxic to normal human cells 31. Pdf evaluating medicinal plants for anticancer activity. Evaluation of traditionally used medicinal plants for anticancer, antioxidant.
Antiinflammatory and anticancer activity of heracleum. Isolation of phytochemicals from anticancer active extracts of. Jun 10, 2012 six herbs in the plant genetics conservation project that have been used as complementary medicines were chosen on the basis of their medicinal value, namely terminalia mucronata, diospyros winitii, bridelia insulana, artabotrys harmandii, terminallia triptera, and croton oblongifolius. This study aims to evaluate the potential anticancer activity of 50% ethanolwater extracts of these six. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. It was found that both tpc and aa are highest for plant extracted with 80% ethanol, followed by water, and finally with 100% ethanol. Evaluation of venezuelan medicinal plant extracts for. Whole plant extracts induce cell death of tumor cells via apoptosis. Numerous reports have identified therapeutic roles for plants and their extracts and constituents.
593 304 644 653 857 1352 407 365 1337 1140 188 388 1548 1609 898 954 1364 422 1365 326 296 1163 1291 25 597 399 1391 575 1057 374 257 602 1126 21 748 584 296 1275 1165 1058 257 1216 986 86 312 247